ABSTRACT
In the past several years,more and more outbreaks of new infectious diseases emerged all over the world and some epidemics seriously affected China.Some kinds of zoonotic pathogens such as West Nile virus,Marburg virus,Lassa virus,Venezuelan equine encephalitis virus,Nipa virus,Trypanosoma brucei,Rift valley fever virus have caused seriously epidemics between human beings and animals.With the great development of the economy and international trade in China,the global spread of infectious diseases should cause great concern in china.
ABSTRACT
<p><b>BACKGROUND</b>To isolate and identify pathogen of atypical pneumonia in Guangdong.</p><p><b>METHODS</b>Pathogens were isolated from variety of samples collected from atypical pneumonia patient by using MDCK cells, and identified with serological and molecular methods.</p><p><b>RESULTS</b>A novel coronavirus was isolated from patients with atypical pneumonia, from which an RNA fragment of 279 nt was amplified by nested RT-PCR. And sequence assay showed that only 39-65 percent of sequence of the virus was homogenous to known coronavirus, but almost 100% homogenous (with one base exception, 12a to t) to SARS-associated coronavirus isolated from patients outside Guangdong, such as in Beijing, Hong Kong, Taiwan, Germany, Italy and so on. Indirect immunofluorescence test showed a specific antigen-antibody reactivity between the coronavirus and convalescent-phase sera of SARS patients.</p><p><b>CONCLUSION</b>The pathogen of the atypical pneumonia in Guangdong province was a novel type of coronavirus, which could be isolated by using MDCK cells.</p>